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IRTG / Soft Matter Science
Freiburger Materialforschungszentrum
Stefan-Meier-Str. 21
79104 Freiburg, Germany


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You are here: Home Events Prof. David Alsteens "Nanomechanical Mapping of Virus Binding Sites to Animal Cells"

Prof. David Alsteens "Nanomechanical Mapping of Virus Binding Sites to Animal Cells"

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Université catholique de Louvain, Croix du sud 4-5, bt L7.07.07, 1348 Louvain-La-Neuve, Belgium

  • Seminar
When Nov 07, 2018
from 02:15 PM to 03:00 PM
Where Seminarraum A, FMF, Stefan-Meier-Str. 21, Freiburg
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Currently, there is a growing need for methods that can quantify and map the molecular interactions of biological samples, both with high-force sensitivity and high spatial resolution. Force-distance (FD) curve-based atomic force microscopy is a valuable tool to simultaneously contour the surface and map the biophysical properties of biological samples at the nanoscale. We will report the use of advanced FD-based technology combined with chemically functionalized tips to probe the localization and interactions of chemical and biological sites on single native proteins and on living cells at high-resolution. I will present how an atomic force and confocal microscopy set-up allows the surface receptor landscape of cells to be imaged and the virus binding events within the first millisecond of contact with the cell to be mapped at high resolution (<50 nm)1. I will also highlight theoretical approaches to contour the free-energy landscape of early binding events between an engineered virus and cell surface receptors. Owing to its key capabilities (quantitative mapping, resolution of a few nanometers, and true correlation with topography)2-4, this novel biochemically sensitive imaging technique is a powerful complement to other advanced AFM modes for quantitative, high-resolution bioimaging.

1. D. Alsteens et al. Nat. Nanotechnol. 12 (2017) 177-183.
2. D. Alsteens et al. Nat. Rev. Materials 2 (2017) 17008.
3. D. Alsteens et al. Nat. Methods 12 (2015) 845-851.
4. M. Delguste et al. Science Adv 4 (2018) eaat1273

invited by Dr. Ramin Omidvar

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