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You are here: Home Events Prof. Mitchell A. Winnik "Metal-Encoded Nanoparticles and Microbeads for Highly Multiplexed Bioassays Based on Atomic Mass Spectrometry "

Prof. Mitchell A. Winnik "Metal-Encoded Nanoparticles and Microbeads for Highly Multiplexed Bioassays Based on Atomic Mass Spectrometry "

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Department of Chemistry, University of Toronto, Canada

What
  • Seminar
When Jan 25, 2013
from 10:30 AM to 11:30 AM
Where («Amphithéâtre de l’Institut Charles Sadron», 23 rue du Loess, 67034 Strasbourg
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We are interested in biological assays based upon inductively coupled plasma mass spectrometry [ICP-MS], a technique widely used in mining and metallurgy for its ability to measure quantitatively, with high sensitivity, the presence of different metals in a sample. In analogy with fluorescence based bioassays, where one uses quantum dots or fluorescent dyes as labels, we use lanthanide (Ln) metal ions as labels. The focus of today’s presentation is on polymer bead-based assays designed for detection by the new technique of mass cytometry. In this technique, cells or polymer beads are injected individually but stochastically into the plasma torch of a time-of-flight ICP-MS device at a rate of 1000 beads/s. The plasma vaporizes, atomizes, and ionizes the cell or bead and its contents, and is able to take (and analyze) ca. 30 mass spectra of the ion cloud generated from each bead. I will begin the talk by introducing metal chelating polymers attached to antibodies for cell-by-cell immunoassays. I will show how one can obtain quantitative information about the antibody binding capacity of each of the targeted cells in a multiplexed assay. Then I will introduce the topic of highly multiplexed bead-based assays. To develop this method, we used dispersion polymerization in ethanol to synthesize lanthanide-ion-encoded polystyrene copolymer beads (with a very narrow size distribution) containing from 105 to 108 lanthanide ions as encoding elements.
Mass cytometry has many goals in common with flow cytometry. For applications to cell suspensions, it is helpful to have calibration beads mixed with the cells to monitor the plasma and any instrumental drift during the measurement. For bead-based assays, the beads themselves must have appropriate surface functionality to attach bioaffinity agents such as antibodies.
The talk will describe challenges in the particle synthesis, examination of ion leakage upon storage, the use of these beads in model bioassays, and recent advances in improving our ability to attach bioaffinity agents to the particle surface.

 

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